Evaluating the Effects of Enrofloxacin on Angiogenesis Using the Chick Embryo Chorioallantoic Membrane Model

Objective- Because little information was available in the literature about the angiogenic property of enrofloxacin, this investigation was undertaken to evaluate this aspect using an in vivomodel. Design- Experimental study. Animals- Twenty fertile chicken eggs (Ross 308) with the average egg-weight of 55 ± 0.4 g were randomly divided into experimental and control groups. Procedures- Agar pellets were prepared with enrofloxacin concentrations at a dosage of 10 mg/Kg egg-weight. A window was carved on the eggshell and drug-impregnated pellet applied on day 8 of incubation on the surface of the chick chorioallantoic membrane. The response of vascular plexus to the drug application was evaluated. Results- Our results showed that enrofloxacin decrease angiogenesis as shown by a reduction in the morphometric parameters of vascular plexus including total vessel's length, the number of vascular branch, vascular complexity and capillary density (p<0.05). Conclusion and Clinical Relevance- Based on findings, it is suggested that enrofloxacin offer a new class of anti-angiogenic agents that might augment the drugs available for the clinical treatment of angiogenesis related diseases.